I'm not sure if this would count, but one of the first that popped into my mind when reading the title is Infested Kerrigan from Starcraft (Brood Wars in particular). She is a bitch (I think she even states it herself once).
Disclaimer: I haven't yet played Starcraft 2. No spoilers please!
Ivo.
What video game character is the biggest jerk?
Re: What video game character is the biggest jerk?
Clearly Mario is the biggest jerk.